Human/Mouse Sonic Hedgehog N‑Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB464
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 171001
Product Specifications
Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Specificity
Detects the N-terminal peptide of human (amino acids 24-197) and mouse (amino acids 25-198) Shh in direct ELISAs and Western blots. Shows 50-100% cross-reactivity with the N-terminal peptides of recombinant mouse (rm) Dhh (amino acids 23‑198) and rmIhh (amino acids 66‑240) and no cross-reactivity with the C‑terminal peptides from rmShh (amino acids 199‑437), rmDhh (amino acids 199‑396), or rmIhh (amino acids 241‑449).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse Sonic Hedgehog N‑Terminus Antibody
Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot.
Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB464) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Sonic Hedgehog N‑Terminus Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human and mouse brain tissue
Sample: Human and mouse brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sonic Hedgehog/Shh
Alternate Names
HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS
Gene Symbol
SHH
UniProt
Additional Sonic Hedgehog/Shh Products
Product Documents for Human/Mouse Sonic Hedgehog N‑Terminus Antibody
Product Specific Notices for Human/Mouse Sonic Hedgehog N‑Terminus Antibody
For research use only
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