Human Oct-4A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB17591
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 653108
Product Specifications
Immunogen
E. coli-derived recombinant human Oct-4A
Met1-Glu135
Accession # NP_002692
Met1-Glu135
Accession # NP_002692
Specificity
Detects human Oct-4A in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Oct-4A Antibody
Detection of Human Oct‑4A by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Oct-4A at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Oct-4A and E-Cadherin in BG01V Human Stem Cells.
Oct-4A and E-Cadherin were detected in human BG01V embryonic stem cells grown on irradiated MEF cells using 10 µg/mL Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) and 10 µg/mL Human E-Cadherin Affinity-purified Polyclonal Antibody (AF648). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Oct-4A using the Northern-Lights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009), and stained for E-Cadherin using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001). Specific staining of Oct-4A was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Oct‑4A in BG01V Human Stem Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Human Oct-4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human Oct-4A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines
Sample: Immersion fixed BG01V human embryonic stem cells and immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: BG01V human embryonic stem cells or iPSCs fixed with paraformaldehyde and permeabilized with saponin
Sample: BG01V human embryonic stem cells or iPSCs fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line
Reviewed Applications
Read 2 reviews rated 4 using MAB17591 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Oct-4A
Long Name
Octamer-binding Transcription Factor 4A
Alternate Names
Oct4A
Gene Symbol
POU5F1
UniProt
Additional Oct-4A Products
Product Documents for Human Oct-4A Antibody
Product Specific Notices for Human Oct-4A Antibody
For research use only
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