Detects human PD-1 in direct ELISA. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Pembrolizumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG₄ kappa

Scientific Data Images for Human PD-1 (Research Grade Pembrolizumab Biosimilar) Antibody

Detection of PD-1 in HEK 293 cells by Flow Cytometry.

HEK 293 cells transfected with eGFP and hPD-1 (A) or irrelevant protein (B) were stained with Human Anti-Human PD-1 (Research Grade PembrolizumabBiosimilar) Monoclonal Antibody (Catalog # MAB11380) followed by Goat Anti-Human IgG Allophycocyanin (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PD-1 (Research Grade Pembrolizumab Biosimilar) Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK 293 cells transfected with eGFP and hPD-1 or irrelevant protein

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-1

Pembrolizumab is a humanized monoclonal IgG4 kappa PD-1 blocking antibody. PD-1 (programmed death receptor-1) is expressed on the surface of lymphocytes, and is also considered an immune checkpoint. The binding of the antibody with PD-1 interferes with the interaction with its ligands PD-L1 and PD-L2 preventing the inhibition of TCR-mediated T-cell proliferation. The inhibition of this PD-1 pathway results in enhanced immunosurveillance and anti-tumor immune response. The original therapeutic has been approved by the FDA for refractory melanoma, as well as other types of cancers such as non-small cell lung cancer, cervical cancer, head and neck cancer and Hodgkin's lymphoma.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Entrez Gene IDs

5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)

Gene Symbol

PDCD1

Additional PD-1 Products

Product Documents for Human PD-1 (Research Grade Pembrolizumab Biosimilar) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PD-1 (Research Grade Pembrolizumab Biosimilar) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human PD-1 (Research Grade Pembrolizumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11380

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PD-1 Antibodies »

Product Specifications

Immunogen

Specificity