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Human PD-L1/B7-H1 (Research Grade Durvalumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10355

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems, part of Bio-Techne
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MAB10355-100
MAB10355-SP

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu125

Product Specifications

Immunogen

Human PD-L1/B7-H1

Specificity

Detects human PD-L1/B7-H1 in direct ELISAs.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human PD-L1/B7-H1 (Research Grade Durvalumab Biosimilar) Antibody

Detection of PD-L1/B7-H1 antibody in MDA-MB-231 human breast adenocarcinoma cell line antibody by Flow Cytometry.

Detection of PD-L1/B7-H1 in MDA-MB-231 human breast adenocarcinoma cell line by Flow Cytometry.

MDA-MB-231 human breast adenocarcinoma cell line was stained with Human Anti-Human PD-L1/B7-H1 (Research Grade Durvalumab Biosimilar) Monoclonal Antibody (Catalog # MAB10355, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PD-L1/B7-H1 (Research Grade Durvalumab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MDA-MB-231 human breast adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

Durvalumab is a biosimilar directed against PD-L1/B7-H1.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

Additional PD-L1/B7-H1 Products

Product Documents for Human PD-L1/B7-H1 (Research Grade Durvalumab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PD-L1/B7-H1 (Research Grade Durvalumab Biosimilar) Antibody

For research use only

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