Human PDK-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF864
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
KLH-coupled human PDK-1 synthetic peptide
CKIIKLEYDFPEKFFPKARD
Accession # AAD38505
CKIIKLEYDFPEKFFPKARD
Accession # AAD38505
Specificity
Detects human PDK-1 in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human PDK-1 Antibody
Detection of Human PDK-1 by Western Blot.
Western blot shows lysates of WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human PDK-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF864) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDK-1 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Human PDK-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: WS-1 human fetal skin fibroblast cell line
Sample: WS-1 human fetal skin fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDK-1
Long Name
Phosphoinositide Dependent Kinase-1
Alternate Names
PDK1, PDPK1, PKB kinase
Gene Symbol
PDPK1
UniProt
Additional PDK-1 Products
Product Documents for Human PDK-1 Antibody
Product Specific Notices for Human PDK-1 Antibody
For research use only
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