Human PDK-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF864

R&D Systems, part of Bio-Techne
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AF864
AF864-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all PDK-1 Antibodies »

Product Specifications

Immunogen

KLH-coupled human PDK-1 synthetic peptide
CKIIKLEYDFPEKFFPKARD
Accession # AAD38505

Specificity

Detects human PDK-1 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human PDK-1 Antibody

Detection of Human PDK-1 antibody by Western Blot.

Detection of Human PDK-1 by Western Blot.

Western blot shows lysates of WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human PDK-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF864) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDK-1 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human PDK-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: WS-1 human fetal skin fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDK-1

PDK-1 (Phosphoinositide-dependent Kinase-1), also called PKB kinase and PDPK1, is a member of the AGC group of serine/threonine kinases. Following insulin, growth factor, or pervanadate stimulation, PDK-1 phosphorylates and activates kinases from the Akt, p70 S6K, and RSK families, and is therefore involved in the regulation of cell proliferation and apoptosis.

Long Name

Phosphoinositide Dependent Kinase-1

Alternate Names

PDK1, PDPK1, PKB kinase

Entrez Gene IDs

5170 (Human); 18607 (Mouse); 81745 (Rat)

Gene Symbol

PDPK1

UniProt

AAD38505

Additional PDK-1 Products

Product Documents for Human PDK-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PDK-1 Antibody

For research use only

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