Human Phospho-EGFR (Y1197) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8274
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 869286
Product Specifications
Immunogen
Phosphopeptide containing the human EGFR Y1197 site
Accession # P00533
Accession # P00533
Specificity
Detects human EGFR when phosphorylated at Y1197 in direct ELISAs and Western blots. For Western blot applications, Catalog # MAB8058 is recommended.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human Phospho-EGFR (Y1197) Antibody
Phospho-EGFR (Y1197) in A431 Human Cell Line.
EGFR phosphorylated at Y1197 was detected in immersion fixed A431 human epithelial carcinoma cell line treated (upper panel) or not (lower panel) with EGF (Catalog # 236-EG) using Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8274) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Phospho-EGFR (Y1197) by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8274) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y1197) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.For Western blot applications, Catalog # MAB8058 is recommended.Applications for Human Phospho-EGFR (Y1197) Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with EGF
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with EGF
Western Blot
For Western blot applications, Catalog # MAB8058 is recommended.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGFR
Long Name
Epidermal Growth Factor Receptor
Alternate Names
EGF R, ErbB, ErbB1, HER-1
Gene Symbol
EGFR
UniProt
Additional EGFR Products
Product Documents for Human Phospho-EGFR (Y1197) Antibody
Product Specific Notices for Human Phospho-EGFR (Y1197) Antibody
For research use only
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