Human Phospho-LAT (Y161) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6334
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 627945
Product Summary for Human Phospho-LAT (Y161) Antibody
Immunogen
Phosphopeptide containing the human LAT Y161 site
Accession # O43561
Accession # O43561
Specificity
Detects human Phospho-LAT (Y161) in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospho-LAT (Y161) Antibody
Detection of Human Phospho-LAT (Y161) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Pervanadate (PV) for 5 minutes. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-LAT (Y161) Monoclonal Antibody (Catalog # MAB6334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-LAT (Y161) at approximately 40 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 1 µg/mL of Sheep Anti-Human LAT Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF6208). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-LAT (Y161) Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAT
LAT (Linker for Activation of T cells) is a type III transmembrane lipid raft protein that serves as a scaffold for signaling molecules. Isoforms of 262 (36 kDa) and 233, called long and short, respectively, differ by inclusion/exclusion of aa 114‑142; a 269 aa isoform lacks this region, but includes alternate N-terminal sequence. Upon T-cell antigen receptor activation, LAT is multiply phosphorylated by ZAP-70/Syk protein tyrosine kinases, creating docking sites for SH2 domain-containing proteins. Phospholipase C-gamma docks at Y161, which is pY132 in the short form of LAT. Mutation of this site results in a Th2 autoimmune lymphoproliferative disorder in mice.
Long Name
Linker for Activation of T cells
Alternate Names
pp36
Gene Symbol
LAT
UniProt
Additional LAT Products
Product Documents for Human Phospho-LAT (Y161) Antibody
Product Specific Notices for Human Phospho-LAT (Y161) Antibody
For research use only
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