Human Phospho-Nbs1 (S343) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4944
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human Nbs1 S343 site
Specificity
Detects human Nbs1 when phosphorylated at S343. Does not recognize Nbs1 when unphosphorylated at S343.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-Nbs1 (S343) Antibody
Detection of Human Phospho-Nbs1 (S343) by Western Blot.
Western blot shows Nbs1 immunoprecipitated from lysates of HeLa human cervical epithelial carcinoma cell line using Human Nbs1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1573). HeLa cell line was mock treated or exposed to 100 J/m2UV-C for 3 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-Nbs1 (S343) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4944) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Nbs1 (S343) was detected at approximately 95 kDa (as indicated). For additional reference the membrane was stripped and reprobed with 1 µg/mL Human/Mouse/Rat Nbs1 Monoclonal Antibody (lower panel, Catalog # MAB1573) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-Nbs1 (S343) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Phospho-Nbs1 (S343) immunoprecipitated from lysates of HeLa human cervical epithelial carcinoma cell line exposed to UV-C using Human Nbs1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1573)
Sample: Phospho-Nbs1 (S343) immunoprecipitated from lysates of HeLa human cervical epithelial carcinoma cell line exposed to UV-C using Human Nbs1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1573)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nbs1
The Nijmegen Breakage Syndrome 1 (Nbs1) protein is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. Nbs1 is phosphorylated at S343 by ATM in response to double-strand breaks and by ATR under replication stress. This phosphorylation triggers the inactivation of late origin firing, which is essential for mediating the intra-S-phase checkpoint. Mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome.
Long Name
Nijmegen Breakage Syndrome 1
Alternate Names
NBN, Nibrin, p95
Gene Symbol
NBN
Additional Nbs1 Products
Product Documents for Human Phospho-Nbs1 (S343) Antibody
Product Specific Notices for Human Phospho-Nbs1 (S343) Antibody
For research use only
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