Human Phospho-PGC1 alpha (S571) Antibody

PGC-1 alpha (PPAR-gamma coactivator 1; also LEM6) is a 97-120 kDa member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC-1 alpha participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPAR gamma, RAR and TR. Human PCG-1 alpha is 798 amino acids (aa) in length. It contains an LxxLL nuclear receptor binding motif (aa 144-148), one PPAR-gamma interaction domain (aa 293-339), two NLSs and an RNA binding/processing region (aa 566-710). PGC-1 alpha activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC-1 alpha activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG-1 alpha acetylation that promotes PGC-1 alpha dissociation from target gene promoters.