Human PRAS40 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6408
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 660928
Product Specifications
Immunogen
E. coli-dervied recombinant human PRAS40
Gln119-Tyr256
Accession # Q96B36
Gln119-Tyr256
Accession # Q96B36
Specificity
Detects human PRAS40 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PRAS40 Antibody
Detection of Human PRAS40 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRAS40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PRAS40 by Simple WesternTM.
Simple Western lane view shows lysates of 293T human embryonic kidney cell line, loaded at 0.5 mg/mL. A specific band was detected for PRAS40 at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
PRAS40 in HeLa Human Cell Line.
PRAS40 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human PRAS40 Monoclonal Antibody (Catalog # MAB6408) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PRAS40 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Simple Western
10 µg/mL
Sample: 293T human embryonic kidney cell line
Sample: 293T human embryonic kidney cell line
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, MDA‑MB‑468 human breast cancer cell line, and T47D human breast cancer cell line
Sample: 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, MDA‑MB‑468 human breast cancer cell line, and T47D human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRAS40
Long Name
40 kDa Proline-rich Akt1 Substrate
Alternate Names
AKT1S1, Lobe
Gene Symbol
AKT1S1
UniProt
Additional PRAS40 Products
Product Documents for Human PRAS40 Antibody
Product Specific Notices for Human PRAS40 Antibody
For research use only
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