Human Pro Caspase-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6864
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Pro Caspase-10
Met1-Pro228
Accession # Q92851
Met1-Pro228
Accession # Q92851
Specificity
Detects human Pro Caspase-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Caspase-2, rhCaspase-3 (long and short forms), rhCaspase-3, rhCaspase-7, rhCaspase-8, rhCaspase-8 p10, rhCaspase-8 p20 and rhCaspase-9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Pro Caspase-10 Antibody
Detection of Human Pro Caspase‑10 by Western Blot.
Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line, human heart tissue, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Pro Caspase-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pro Caspase-10 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Pro Caspase‑10 in Jurkat Human Cell Line.
Pro Caspase-10 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human Pro Caspase-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6864) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Pro Caspase-10 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line
Western Blot
1 µg/mL
Sample: KG‑1 human acute myelogenous leukemia cell line, human heart tissue, and Jurkat human acute T cell leukemia cell line
Sample: KG‑1 human acute myelogenous leukemia cell line, human heart tissue, and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-10
Caspase-10 (Cysteine-aspartic acid protease 10, FLICE2 and Mch4) is a 28-29 kDa member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on procaspases-3, 4, 6, 7, and 9. Human procaspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43 generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.
Alternate Names
CASP10, Caspase10, FLICE2, Mch4
Entrez Gene IDs
843 (Human)
Gene Symbol
CASP10
UniProt
Additional Caspase-10 Products
Product Documents for Human Pro Caspase-10 Antibody
Product Specific Notices for Human Pro Caspase-10 Antibody
For research use only
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