Human Relaxin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2804
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 349102
Product Specifications
Immunogen
E. coli-derived recombinant human Relaxin-2
Val23-Cys185
Accession # P04090
Val23-Cys185
Accession # P04090
Specificity
Detects both the pro and mature forms of human Relaxin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Relaxin-1 or rhRelaxin-3 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Relaxin-2 Antibody
Detection of Human Relaxin-2 by Western Blot.
Western blot shows lysates of human placenta. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Relaxin-2 Monoclonal Antibody (Catalog # MAB2804) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Relaxin-2 at approximately 8 kDa and 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
cAMP production Induced by Relaxin-2 and Neutralization by Human Relaxin-2 Antibody.
Recombinant Human Relaxin-2 (Catalog # 3596-RN) induces cAMP production in the THP-1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line), as measured by cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recombinant Human Relaxin-2 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human Relaxin-2 Monoclonal Antibody (Catalog # MAB2804). The ND50 is typically 1.5-6.0 µg/mL.Applications for Human Relaxin-2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human placenta
Sample: Human placenta
Neutralization
Measured by its ability to neutralize Relaxin-2-induced cAMP production in the THP-1 human acute monocytic leukemia cell line. Parsell, D. A. et al. (1996) J. Biol. Chem. 271:27936. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 7.5 ng/mL Recombinant Human Relaxin-2.
Reviewed Applications
Read 1 review rated 5 using MAB2804 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Relaxin-2
Human Relaxin-2/H2 is a 6 kDa member of the insulin/relaxin gene superfamily. Relaxin-2 is synthesized as a 185 aa preprohormone that is proteolytically processed into a nonglycosylated, disulfide-linked, 53 aa mature heterodimer that contains a 29 aa B chain (aa 25‑53) and a 24 aa A chain (aa 161‑185). An alternate splice form of human Relaxin-2 is known that contains the B chain and an uncleaved 47 aa extension. Mature human Relaxin-2 heterodimer is 48%, 44% and 43% aa identical to rat, canine and porcine Relaxin-2, respectively.
Additional Relaxin-2 Products
Product Documents for Human Relaxin-2 Antibody
Product Specific Notices for Human Relaxin-2 Antibody
For research use only
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