Human RSK4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3916
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 374521
Product Specifications
Immunogen
E. coli-derived recombinant human RSK4
Met1-Leu745
Accession # Q9UK32
Met1-Leu745
Accession # Q9UK32
Specificity
Detects human RSK4 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) RSK1, rhRSK2, or rhRSK3 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human RSK4 Antibody
Detection of Human RSK4 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human RSK4 by Western Blot.
Western blot shows Recombinant Human Active RSK1 (Catalog # 992-KS), recombinant human RSK2, recombinant human RSK3, and recombinant human RSK4 (2 ng/lane). PVDF membrane was probed with 2 µg/mL Rat Anti-Human RSK4 Monoclonal Antibody (Catalog # MAB3916) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for RSK4 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human RSK4 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RSK4
The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases frequently activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSK4, also known as RPS6KA6, is commonly deleted in patients with complex X-linked mental retardation. Unlike other RSKs, RSK4 is constitutively active in serum-starved cells, and may participate in non-growth factor signaling.
Long Name
Ribosomal Protein S6 Kinase IV
Alternate Names
RPS6KA6
Gene Symbol
RPS6KA6
UniProt
Additional RSK4 Products
Product Documents for Human RSK4 Antibody
Product Specific Notices for Human RSK4 Antibody
For research use only
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