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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 246523

Product Specifications

Immunogen

E. coli-derived recombinant human STAT1
Met1-Gln194
Accession # P42224

Specificity

Detects human STAT1. Detects recombinant human STAT1 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human STAT1 Alexa Fluor® 647-conjugated Antibody

Detection of STAT1 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of STAT1 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human STAT1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC1490R, filled histogram) or isotype control antibody (Catalog # IC013R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human STAT1 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT1

STAT1 is a member of the STAT family of cytoplasmic transcription factors that mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1a at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human); 20846 (Mouse)

Gene Symbol

STAT1

UniProt

Additional STAT1 Products

Product Documents for Human STAT1 Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human STAT1 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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