Human STAT1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1490
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry
Cited:
Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 246523
Product Specifications
Immunogen
E. coli-derived recombinant human STAT1
Met1-Gln194
Accession # P42224
Met1-Gln194
Accession # P42224
Specificity
Detects human STAT1. Detects recombinant human STAT1 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human STAT1 Antibody
STAT1 in HeLa Human Cell Line.
STAT1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human STAT1 Monoclonal Antibody (Catalog # MAB1490) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of STAT1 in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human STAT1 Monoclonal Antibody (Catalog # MAB1490, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human STAT1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using MAB1490 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT1
Long Name
Signal Transducer and Activator of Transcription 1
Alternate Names
CANDF7, ISGF-3, STAT91
Gene Symbol
STAT1
UniProt
Additional STAT1 Products
Product Documents for Human STAT1 Antibody
Product Specific Notices for Human STAT1 Antibody
For research use only
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