HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human STAT1 APC-conjugated Monoclonal Antibody (Catalog # IC1490A, filled histogram) or isotype control antibody (Catalog # IC013A, open histo-gram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human STAT1 APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT1

STAT1 is a member of the STAT family of cytoplasmic transcription factors that mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1a at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human); 20846 (Mouse)

Gene Symbol

STAT1

UniProt

P42224

Additional STAT1 Products

Product Documents for Human STAT1 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human STAT1 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human STAT1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1490A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all STAT1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human STAT1 APC-conjugated Antibody

Detection of STAT1 in HeLa Human Cell Line by Flow Cytometry.