Human T-bet/TBX21 MAb (Clone 525831)
R&D Systems, part of Bio-Techne | Catalog # MAB53851
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 525831
Product Specifications
Immunogen
E. coli-derived recombinant human T‑bet/TBX21
Glu326-Asn535
Accession # Q9UL17
Glu326-Asn535
Accession # Q9UL17
Specificity
Detects human T‑bet/TBX21 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human T-bet/TBX21 MAb (Clone 525831)
Detection of T-bet/TBX21 in Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 µg/mL) and 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) for 5 days to induce Th1 cell development were stained with Mouse Anti-Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB53851) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).Applications for Human T-bet/TBX21 MAb (Clone 525831)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 μg/mL) and Recombinant Human IL‑12 (Catalog # 219-IL;10 ng/mL) for 5 days to induce Th1 cell development were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 μg/mL) and Recombinant Human IL‑12 (Catalog # 219-IL;10 ng/mL) for 5 days to induce Th1 cell development were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB53851 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: T-bet/TBX21
T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1 cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Long Name
T-box transcription factor TBX21
Alternate Names
T-PET, Tbet, TBLYM, TBX21
Gene Symbol
TBX21
UniProt
Additional T-bet/TBX21 Products
Product Documents for Human T-bet/TBX21 MAb (Clone 525831)
Product Specific Notices for Human T-bet/TBX21 MAb (Clone 525831)
For research use only
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