Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Biotin

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu7

View all TNF-alpha Antibodies »

Product Specifications

Immunogen

Human TNF‑ alpha

Specificity

Detects human TNF- alpha in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Adalimumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images

Detection of TNF- alpha antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TNF‑ alpha in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) (A) treated with PMA and Ca2+ ionomycin overnight or (B) resting, were stained with Human Anti-Human TNF- alpha (Research Grade Adalimumab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9677B) followed by APC-conjugated Streptavidin (Catalog # F0050) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ ionomycin fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF-alpha

Adalimumab is a biosimilar directed against the extracellular domain of TNF- alpha. The biotinylated antibody can be used in flow cytometry or various assay format to measure TNF- alpha expression and can be detected by conjugated streptavidin.

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)

Gene Symbol

TNF

Additional TNF-alpha Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices

For research use only

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