Human TRAIL/TNFSF10 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF375
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Canine, Feline
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Affinity Purification, Bioassay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL/TNFSF10
Thr95-Gly281
Accession # P50591
Thr95-Gly281
Accession # P50591
Specificity
Detects human TRAIL/TNFSF10 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRAIL is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TRAIL/TNFSF10 Antibody
Detection of Human TRAIL/TNFSF10 by Western Blot.
Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TRAIL/TNFSF10 at approximately 27 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TRAIL/TNFSF10 in Human Brain.
TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
TRAIL/TNFSF10 in Human Prostate Cancer Tissue.
TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human TRAIL/TNFSF10 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (occipital cortex) and immersion fixed paraffin-embedded sections of human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human brain (occipital cortex) and immersion fixed paraffin-embedded sections of human prostate cancer tissue
Western Blot
1 µg/mL
Sample: 786‑O human renal cell adenocarcinoma cell line
Sample: 786‑O human renal cell adenocarcinoma cell line
Neutralization
Measured by its ability to neutralize TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically <140 ng/mL in the presence of 12 ng/mL Recombinant Human TRAIL/TNFSF10, cross-linking Anti-Mouse His Tag Monoclonal Antibody and Actinomycin D.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL/TNFSF10
Long Name
TNF-related Apoptosis-inducing Ligand
Alternate Names
CD253, TNFSF10
Gene Symbol
TNFSF10
UniProt
Additional TRAIL/TNFSF10 Products
Product Documents for Human TRAIL/TNFSF10 Antibody
Product Specific Notices for Human TRAIL/TNFSF10 Antibody
For research use only
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