Human TROP-2 Antibody

Detection of Human TROP-2 by Immunohistochemistry

Downregulation of Trop2 inhibits cell invasion. (A) The invasive capability of Hep2 cells transfected with NC or Trop2 siRNA (Trop2-S1) was measured at the indicated time points using the Transwell assay. (B) The number of cells on the underside of the chamber was counted. NC, negative control, siRNA, small interfering RNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25779928), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TROP-2 by Western Blot

Knockdown of Trop2 expression in Hep2 cells by siRNA. (A) Trop2 mRNA expression in Hep2 cells was examined by reverse transcription-quantitative polymerase chain reaction 48 h after transfection with Trop2 siRNA or NC, as indicated (*P<0.05, **P<0.001). (B) Western blot analysis of Trop2 protein expression in Hep2 cells 48 h following transfection with siRNA as indicated. siRNA, small interfering RNA; NC, negative control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25779928), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TROP-2 by Knockdown Validated

Trop2 Loss Promotes ErbB3 Activation in HNSCC CellsA. Immunofluorescence images of Trop2 staining (green) in SCC1 HNSCC cells after stable knockdown using short hairpins targeting the Trop2 cDNA. Nuclei are counterstained with 4′6-diamidino-2-phenylindole (DAPI). B. Results of a phosphorylated receptor tyrosine kinase antibody array demonstrating elevated p-ErbB3 in lysates from Trop2 knockdown SCC1 cells. The exposures were normalized to the control spots (four corners), which exhibit equal intensities. C&D. Representative immunoblots showing hyperactivation of ErbB3 and AKT caused by Trop2 loss in SCC1 and SCC25 HNSCC cells. Two short hairpins targeting distinct regions of the Trop2 cDNA were used. E. Reduction of ErbB3 activity after ectopic expression of an RNAi-resistant Trop2 cDNA in Trop2 knockdown SCC1 cells. Arrow points to the lower band which is the correct size for Trop2. F. Ectopic expression of a Flag-epitope tagged Trop2 cDNA in SCC1 cells suppresses basal ErbB3 and AKT activation. Control is an empty vector. Relative increases in phosphoproteins in control versus experimental groups were quantified by photodensitometry after normalization to total ErbB3 or AKT protein which served as an internal controls. Immunoblots are representative of at least three independent experiments. Significance was measured by student's t test, * (P<0.05), ** (P<0.01), *** (P<0.001). Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.2423), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TROP-2 by Western Blot

Trop2 expression in laryngeal squamous cell carcinoma tissue. (A) Western blot analysis of the protein expression of Trop2 in laryngeal carcinoma tissues (c1, c2, c3 and c4) and paired paracancerous tissues (p1, p2, p3 and p4). Actin was used as a loading control. Representative (B) negative and (C) high expression of Trop2 in paraffin embedding laryngeal carcinoma and precancerous tissues, demonstrated using immunohistochemical staining. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25779928), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TROP-2 by Knockdown Validated

Trop2 Loss Promotes ErbB3 Activation in HNSCC CellsA. Immunofluorescence images of Trop2 staining (green) in SCC1 HNSCC cells after stable knockdown using short hairpins targeting the Trop2 cDNA. Nuclei are counterstained with 4′6-diamidino-2-phenylindole (DAPI). B. Results of a phosphorylated receptor tyrosine kinase antibody array demonstrating elevated p-ErbB3 in lysates from Trop2 knockdown SCC1 cells. The exposures were normalized to the control spots (four corners), which exhibit equal intensities. C&D. Representative immunoblots showing hyperactivation of ErbB3 and AKT caused by Trop2 loss in SCC1 and SCC25 HNSCC cells. Two short hairpins targeting distinct regions of the Trop2 cDNA were used. E. Reduction of ErbB3 activity after ectopic expression of an RNAi-resistant Trop2 cDNA in Trop2 knockdown SCC1 cells. Arrow points to the lower band which is the correct size for Trop2. F. Ectopic expression of a Flag-epitope tagged Trop2 cDNA in SCC1 cells suppresses basal ErbB3 and AKT activation. Control is an empty vector. Relative increases in phosphoproteins in control versus experimental groups were quantified by photodensitometry after normalization to total ErbB3 or AKT protein which served as an internal controls. Immunoblots are representative of at least three independent experiments. Significance was measured by student's t test, * (P<0.05), ** (P<0.01), *** (P<0.001). Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.2423), licensed under a CC-BY license. Not internally tested by R&D Systems.