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Human Tyk2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3427

R&D Systems, part of Bio-Techne
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MAB3427
MAB3427-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 307214

Product Specifications

Immunogen

E. coli-derived recombinant human Tyk2
Pro2-Leu51
Accession # P29597

Specificity

Detects human Tyk2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human Tyk2 Antibody

Detection of Human Tyk2 antibody by Western Blot.

Detection of Human Tyk2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Tyk2 Monoclonal Antibody (Catalog # MAB3427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tyk2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human Tyk2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 3 reviews rated 4 using MAB3427 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyk2

Tyk2 belongs to the Janus family of intracellular tyrosine kinases that are activated by receptors for many cytokines. Activation of Tyk2 occurs via phosphorylation at two adjacent tyrosine residues, Y1054 and Y1055, within the kinase domain. Tyk2 is required for interferon beta-mediated tyrosine phosphorylation of STAT3 and interferon receptor 1. Mice lacking Tyk2 are susceptible to parasitic infection but resistant to autoimmune arthritis.

Long Name

Protein tyrosine kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JTK1, non-receptor tyrosine-protein kinase TYK2, tyrosine kinase 2

Entrez Gene IDs

7297 (Human); 54721 (Mouse); 298706 (Rat)

Gene Symbol

TYK2

UniProt

Additional Tyk2 Products

Product Documents for Human Tyk2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Tyk2 Antibody

For research use only

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