Human XCR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8571
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1097A
Product Specifications
Immunogen
E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094
Specificity
Detects human XCR1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human XCR1 Antibody
Detection of XCR1 in CD141+and HLA-DR+Human Blood Monocytes by Flow Cytometry.
CD141+and HLA-DR+human peripheral blood monocytes were isolated by staining with (A) Mouse Anti-Human HLA-DR Fluorescein-Conjugated Monoclonal Antibody (Catalog # FAB4869F) and Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog # FAB3947A). Isolated cells were then stained with (B) Rabbit Anti-Human XCR1 Monoclonal Antibody (Catalog # MAB8571, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).Applications for Human XCR1 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XCR1
XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells, NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.
Long Name
Chemokine [C Motif] Receptor 1
Alternate Names
CCXCR1, GPR5
Gene Symbol
XCR1
UniProt
Additional XCR1 Products
Product Documents for Human XCR1 Antibody
Product Specific Notices for Human XCR1 Antibody
For research use only
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