IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81040
Recombinant Monoclonal Antibody
![Western Blot: IL-18/IL-1F4 Antibody (125-2H)ChimericAzide and BSA Free [NBP2-81040]](https://resources.bio-techne.com/images/products/IL-18-IL-1F4-Antibody-125-2H-Western-Blot-NBP2-81040-img0001.jpg "Western Blot: IL-18/IL-1F4 Antibody (125-2H)ChimericAzide and BSA Free [NBP2-81040]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 125-2H
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion of splenocytes from mice immunized with human IL-18/IL-1F4, with SP2/O mouse myeloma cells.
Specificity
Recognizes the active form of human IL-18/IL-1F4.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free
Western Blot: IL-18/IL-1F4 Antibody (125-2H)ChimericAzide and BSA Free [NBP2-81040]
Western Blot: IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040] - Western Blot using IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040]. HeLa cell lysate samples (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H [NBP2-81040] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. NBP2-81040 successfully detected IL18 in HeLa cell lysate samples.Applications for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: IL-18/IL-1F4
Long Name
Interleukin 18
Alternate Names
IGIF, IL-1F4, IL-1g, IL18
Gene Symbol
IL18
Additional IL-18/IL-1F4 Products
Product Documents for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free
Product Specific Notices for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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