Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20633
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # IDH1.R132H/7328R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 1-200) of human Isocitrate Dehydrogenase 1/IDH1 (exact sequence is proprietary)
Localization
Cytoplasm. Nucleus.
Specificity
Isocitrate Dehydrogenase 1/IDH1 R132H antibody binds to Isocitrate Dehydrogenase 1/IDH1-mutated protein, but does not bind the wild-type Isocitrate Dehydrogenase 1/IDH1 protein Isocitrate Dehydrogenase 1/IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide Diffuse staining of the fibrillary tumor matrix is also seen
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: HepG2, HeLa, HT29 or MCF7 cells. Human breast, colon or prostate carcinoma.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
FFPE human prostate with IDH1-R132H mutation stained with Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1.R132H/7328R).R132H/7328R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
FFPE human brain with IDH1-R132H mutation stained with Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1.R132H/7328R).R132H/7328R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
1mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Isocitrate Dehydrogenase 1/IDH1
Alternate Names
IDCD, IDH1, IDP, IDPC, PICD
Gene Symbol
IDH1
Additional Isocitrate Dehydrogenase 1/IDH1 Products
- All Products for Isocitrate Dehydrogenase 1/IDH1
- Isocitrate Dehydrogenase 1/IDH1 cDNA Clones
- Isocitrate Dehydrogenase 1/IDH1 ELISA Kits
- Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies
- Isocitrate Dehydrogenase 1/IDH1 Proteins and Enzymes
- Isocitrate Dehydrogenase 1/IDH1 Small Molecules and Peptides
Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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