Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20989
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # IDH1.R132H/7328R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 1-200) of human Isocitrate Dehydrogenase 1/IDH1 (exact sequence is proprietary)
Localization
Cytoplasm. Nucleus.
Specificity
Isocitrate Dehydrogenase 1/IDH1 R132H antibody binds to Isocitrate Dehydrogenase 1/IDH1-mutated protein, but does not bind the wild-type Isocitrate Dehydrogenase 1/IDH1 protein Isocitrate Dehydrogenase 1/IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide Diffuse staining of the fibrillary tumor matrix is also seen
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: HepG2, HeLa, HT29 or MCF7 cells. Human breast, colon or prostate carcinoma.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images
Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
FFPE human prostate with IDH1-R132H mutation stained with Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1.R132H/7328R).R132H/7328R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1.R132H/7328R)
FFPE human brain with IDH1-R132H mutation stained with Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1.R132H/7328R).R132H/7328R). Inset: PBS instead of primary antibody; secondary only negative control.Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
1mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Isocitrate Dehydrogenase 1/IDH1
Alternate Names
IDCD, IDH1, IDP, IDPC, PICD
Gene Symbol
IDH1
Additional Isocitrate Dehydrogenase 1/IDH1 Products
- All Products for Isocitrate Dehydrogenase 1/IDH1
- Isocitrate Dehydrogenase 1/IDH1 cDNA Clones
- Isocitrate Dehydrogenase 1/IDH1 ELISA Kits
- Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies
- Isocitrate Dehydrogenase 1/IDH1 Proteins and Enzymes
- Isocitrate Dehydrogenase 1/IDH1 Small Molecules and Peptides
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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