LDLR Antibody (C7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78159

Novus Biologicals, part of Bio-Techne
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NBP1-78159
NBP1-78159SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine, Bovine

Cited:

Human, Mouse, Porcine, Bovine

Applications

Validated:

Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Proximity Ligation Assay, Radioimmunoassay, Western Blot

Cited:

Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, In vitro assay, Radioimmunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # C7

Concentration

1 mg/ml

View all LDLR Antibodies »

Product Specifications

Immunogen

Partially purified bovine LDL receptor [Swiss-Prot# P01131].

Localization

Cell membrane/cell surface, endomembrane system, endosomes, lysosome, clathrin-coated pit, and Golgi apparatus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for LDLR Antibody (C7)

Western Blot: LDLR Antibody (C7) [NBP1-78159]

Western Blot: LDLR Antibody (C7) [NBP1-78159]

Western Blot: LDL R Antibody (C7) [NBP1-78159] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) [NBP1-78159]

Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) [NBP1-78159]

Immunocytochemistry/Immunofluorescence: LDLR Antibody (C7) [NBP1-78159] - Immunocytochemistry of LA-6 and intact MPEFs (used as positive control) using anti-LDLR antibody. The parental MPEFs reactive to the secondary antibody (fluorescein-labeled anti-mouse IgG) alone are designated as negative control. All cells were counterstained with DAPI upon reaction with the secondary antibody. Bar = 30 um. Image collected and cropped by CiteAb from the following publication (www.mdpi.com/1422-0067/18/12/2610) licensed under a CC-BY license.
Immunohistochemistry: LDLR Antibody (C7) [NBP1-78159]

Immunohistochemistry: LDLR Antibody (C7) [NBP1-78159]

Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining.
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Applications for LDLR Antibody (C7)

Application
Recommended Usage

Electron Microscopy

reported in scientific literature (PMID 11839845)

Flow Cytometry

reported in scientific literature (PMID 10906332)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

reported in scientific literature (PMID 11839845)

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

In vitro assay

reported in scientific literature (PMID 9642270)

Proximity Ligation Assay

reported in scientific literature (PMID 3263645)

Western Blot

1:1000
Application Notes
In WB assay, specific bands can be seen around 160 kDa (mature form) and 120 kDa (precursor) molecular weight positions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-78159 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LDLR

LDL Receptor (low-density lipoprotein receptor or LDLR) is a key determinant of plasma cholesterol levels and as a ubiquitously expressed cell membrane glycoprotein, it binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells via endocytosis. LDLR is found distributed from plasma membrane to intracellular compartments, cell surface (in the presence of PCSK9) and also localizes to Golgi apparatus as well as early/late endosomes and lysosomes. VLDL, IDL, HDL, and chylomicron remnant are also recognized by LDLR at neutral pH. Receptor-ligand complexes undergo endocytosis via clathrin-coated pits and coated vehicle dispenses to endosomes with LRP6 and ARH (also known as LDLR adaptor protein), connecting LDLR family protein and the endocytic machinery; thereby, acidic condition activates dissociation of internalized ligands. Released ligand particles further travel to lysosomes wherein the ligand is degraded by enzyme, while the receptors recycle back to cell surface. After internalization, LDL particles trigger reduction in HMGCR expression to suppress cholesterol biosynthesis; enhancement of ACAT activity to reduce toxic free cholesterol, and suppression of LDLR synthesis to reduce LDL uptake via SREBPs. Genetic mutations impairing LDLR function results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Entrez Gene IDs

3949 (Human); 281276 (Bovine)

Gene Symbol

LDLR

UniProt

P01130, P01131 (Bovine)

Additional LDLR Products

Product Documents for LDLR Antibody (C7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LDLR Antibody (C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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