LDLR Antibody (C7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80833
![Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]](https://resources.bio-techne.com/images/products/LDLR-Antibody-C7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80833-img0002.jpg "Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]")
![Western Blot: LDLR Antibody (C7)Azide and BSA Free [NBP2-80833]](https://resources.bio-techne.com/images/products/LDLR-Antibody-C7-Azide-and-BSA-Free-Western-Blot-NBP2-80833-img0001.jpg "Western Blot: LDLR Antibody (C7)Azide and BSA Free [NBP2-80833]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat, Porcine, Bovine
Applications
Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Radioimmunoassay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # C7
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for LDLR Antibody (C7) - Azide and BSA Free
Immunogen
Partially purified bovine LDL receptor [Swiss-Prot# P01131].
Localization
Cell membrane/cell surface, endomembrane system, endosomes, lysosome, clathrin-coated pit, and Golgi apparatus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for LDLR Antibody (C7) - Azide and BSA Free
Western Blot: LDLR Antibody (C7)Azide and BSA Free [NBP2-80833]
Western Blot: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]](https://resources.bio-techne.com/images/products/LDLR-Antibody-C7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80833-img0003.jpg "Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]")
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]
Immunocytochemistry/Immunofluorescence: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]](https://resources.bio-techne.com/images/products/LDLR-Antibody-C7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80833-img0004.jpg "Immunohistochemistry-Paraffin: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]")
Immunohistochemistry-Paraffin: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833]
Immunohistochemistry-Paraffin: LDLR Antibody (C7) - Azide and BSA Free [NBP2-80833] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining. Image from the standard format of this antibody.Applications for LDLR Antibody (C7) - Azide and BSA Free
Application
Recommended Usage
Electron Microscopy
reported in scientific literature (PMID 11839845)
Flow Cytometry
reported in scientific literature (PMID 10906332)
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200
Immunohistochemistry-Frozen
reported in scientific literature (PMID 11839845)
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
1:10 - 1:500
In vitro assay
reported in scientific literature (PMID 9642270)
Western Blot
1:1000
Application Notes
In WB assay, specific bands can be seen around 160 kDa (mature form) and 120 kDa (precursor) molecular weight positions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine, 0.15M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LDLR
Long Name
Low Density Lipoprotein Receptor
Alternate Names
LDL R
Gene Symbol
LDLR
Additional LDLR Products
Product Documents for LDLR Antibody (C7) - Azide and BSA Free
Product Specific Notices for LDLR Antibody (C7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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