MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72568

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Porcine

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1A5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MAdCAM-1 Antibodies »

Product Summary for MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human MADCAM1(NP_570116) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free

Western Blot: MAdCAM-1 Antibody (OTI1A5)Azide and BSA Free [NBP2-72568]

Western Blot: MAdCAM-1 Antibody (OTI1A5)Azide and BSA Free [NBP2-72568]

Western Blot: MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72568] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM-1.
Immunocytochemistry/ Immunofluorescence: MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72568]

Immunocytochemistry/ Immunofluorescence: MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72568]

Immunocytochemistry/Immunofluorescence: MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72568] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MADCAM1.

Applications for MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAdCAM-1

Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or "homing receptor" for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Gene Symbol

MADCAM1

Additional MAdCAM-1 Products

Product Documents for MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAdCAM-1 Antibody (OTI1A5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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