Mcl-1 Antibody (CL1128)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52968

Novus Biologicals, part of Bio-Techne
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NBP2-52968
NBP2-52968-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1128

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Mcl-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDG

Epitope

PEEELDGYEP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Mcl-1 Antibody (CL1128)

Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968]

Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968]

Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MCL1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of U-2 OS cells using the Anti-MCL1 monoclonal antibody, showing cell cycle dependent staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of lymph node in human colon shows strong positivity in the reaction centrum.
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Applications for Mcl-1 Antibody (CL1128)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-52968 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mcl-1

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Gene Symbol

MCL1

Additional Mcl-1 Products

Product Documents for Mcl-1 Antibody (CL1128)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mcl-1 Antibody (CL1128)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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