Mcl-1 Antibody (CL1128) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43898

Novus Biologicals, part of Bio-Techne
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NBP3-43898
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Catalog #
BSA Free
NBP2-52968
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1128

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Mcl-1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q07820, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Mcl-1 Antibody (CL1128) - Azide and BSA Free

Mcl-1 Antibody (CL1128) - Azide and BSA Free Immunohistochemistry-Paraffin: Mcl-1 Antibody [NBP3-43898]

Immunohistochemistry-Paraffin: Mcl-1 Antibody [NBP3-43898]

Staining of human breast cancer shows strong positivity in tumor cells.
Mcl-1 Antibody (CL1128) - Azide and BSA Free Western Blot: Mcl-1 Antibody [NBP3-43898]

Western Blot: Mcl-1 Antibody [NBP3-43898]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Mcl-1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Mcl-1 Antibody (CL1128) - Azide and BSA Free Immunohistochemistry-Paraffin: Mcl-1 Antibody [NBP3-43898]

Immunohistochemistry-Paraffin: Mcl-1 Antibody [NBP3-43898]

Staining of human cerebellum shows absence of immunoreactivity (negative control).
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Applications for Mcl-1 Antibody (CL1128) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mcl-1

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Gene Symbol

MCL1

Additional Mcl-1 Products

Product Documents for Mcl-1 Antibody (CL1128) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mcl-1 Antibody (CL1128) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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