Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71775

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Gerbil

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 11E9-1H12

Format

BSA Free

Concentration

1 mg/ml

View all Mitofusin 1 Antibodies »

Product Specifications

Immunogen

Mouse Mitofusin 1

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23658809). Human reactivity reported in scientific literature (PMID: 24710686, 24928681).

Localization

Mitochondrion outer membrane; Multi-pass membrane protein

Marker

Mitochondrial Fusion Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Western Blot: Mitofusin 1 Antibody (11E9-1H12)BSA Free [NBP1-71775]

Western Blot: Mitofusin 1 Antibody (11E9-1H12)BSA Free [NBP1-71775]

Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775]

Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775]

Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775]

Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775]

Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 in mouse liver.
View 2 more images

Applications for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500-1:1000
Application Notes
In Western blot, a band can be seen at ~80 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mitofusin 1

Mitochondria are dynamic organelles that continuously divide and fuse, and their dynamics/distribution is critical for their role in bioenergetics and cell survival. Mitochondrial fusion process is mediated by mitofusin 1 (MFN1) and mitofusin 2 (MFN2) proteins in the outer membrane as well as OPA1 in the inner mitochondrial membrane. MFN1 is an essential transmembrane GTPase critical to mitochondrial fusion; acts independently of the cytoskeleton, and its overexpression induces formation of mitochondrial networks. MFN1 forms homo-/-heteromultimers (with MFN2), and the multimerization has been proposed to be essential for mitochondrion fusion. MFN1 interacts with VAT1 and is ubiquitinated by MARCH5. A mutation in the GTPase domain disrupts the mitochondrial fusion activity of MFN1/MFN2 and loss of MFN1/2 leads to mitochondrial dysfunction mediated embryonic lethality in mice. MFN proteins have been reported to regulate mitochondrial membrane potential, while silencing of MFN1/2 resulte in mitochondrial fragmentation and increased apoptotic sensitivity.

Alternate Names

hfzo1, hfzo2, MFN1

Entrez Gene IDs

55669 (Human)

Gene Symbol

MFN1

Additional Mitofusin 1 Products

Product Documents for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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