Mouse CCL19/MIP-3 beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB880
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
ELISA Development (Capture), Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 87102
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta
Gly26-Val107-Leu-Glu (Ser108LeuGlu)
Accession # Q548P0
Gly26-Val107-Leu-Glu (Ser108LeuGlu)
Accession # Q548P0
Specificity
Detects mouse CCL19/MIP‑3 beta in ELISAs and Western blots. In Western blots, no cross-reactivity with
recombinant human
CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,
26, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10, 11, 12, 17, 20, 21, 22,
24, 25, 27 or recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL19/MIP-3 beta Antibody
Chemotaxis Induced by CCL19/MIP‑3 beta and Neutral-ization by Mouse CCL19/MIP‑3 beta Antibody.
Recombinant Mouse CCL19/ MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB880). The ND50 is typically 3-15 µg/mL.Applications for Mouse CCL19/MIP-3 beta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3)
under non-reducing conditions only
Sample: Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CCL19/MIP‑3 beta-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 50 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.
Mouse CCL19/MIP-3 beta Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL19/MIP-3 beta
CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a beta chemokine that binds specifically to the chemokine receptor CCR7/EBI-1/BLR-2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant CCL19 has been shown to be chemotactic for T-cells and B-cells. The CCL19 receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up‑regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
References
- Kim, C.H. et al. (1998) J. Immunol. 160:2418.
- Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
- Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
- Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Alternate Names
ELC, MIP-3 beta, MIP3 beta
Gene Symbol
CCL19
UniProt
Additional CCL19/MIP-3 beta Products
Product Documents for Mouse CCL19/MIP-3 beta Antibody
Product Specific Notices for Mouse CCL19/MIP-3 beta Antibody
For research use only
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