Mouse CCL19/MIP‑3 beta Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF880
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
ELISA Development (Detection), Immunohistochemistry, Immunohistochemistry-Frozen
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta (R&D Systems, Catalog # 440-M3)
Gly26-Val107
Accession # Q548P0
Gly26-Val107
Accession # Q548P0
Specificity
Detects mouse CCL19/MIP-3 beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) TECK, rmTARC, recombinant human MIP-3 beta, rmMIP-1 alpha, rmMIP-1 beta, rm6Ckine, and rmRANTES is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse CCL19/MIP‑3 beta Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3)
Sample: Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3)
Mouse CCL19/MIP-3 beta Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL19/MIP-3 beta
CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse MIP-3 beta cDNA encodes a 108 amino acid residue precursor protein with a predicted 25 aa residue signal peptide that is cleaved to form the 83 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B-cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.
References
- Kim, C.H. et al. (1998) J. Immunol. 160:2418.
- Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
- Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
- Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Alternate Names
ELC, MIP-3 beta, MIP3 beta
Gene Symbol
CCL19
UniProt
Additional CCL19/MIP-3 beta Products
Product Documents for Mouse CCL19/MIP‑3 beta Biotinylated Antibody
Product Specific Notices for Mouse CCL19/MIP‑3 beta Biotinylated Antibody
For research use only
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