Mouse CCL19/MIP-3 beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF880
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Neutralization, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta
Gly26-Val107 (Ser108LeuGlu)
Accession # Q548P0
Gly26-Val107 (Ser108LeuGlu)
Accession # Q548P0
Specificity
Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL19/MIP-3 beta Antibody
Chemotaxis Induced by CCL19/MIP-3 beta and Neutralization by Mouse CCL19/MIP-3 beta Antibody.
Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 µg/mL.
CCL19/MIP‑3 beta in Mouse Splenocytes.
CCL19/MIP-3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CCL19/MIP-3 beta in Mouse Dendritic Cells.
CCL19/MIP-3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse CCL19/MIP-3 beta Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes treated with PHA and immersion fixed mouse dendritic cells
Sample: Immersion fixed mouse splenocytes treated with PHA and immersion fixed mouse dendritic cells
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3)
Sample: Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3)
Neutralization
Measured
by its ability to neutralize CCL19/MIP‑3 beta-induced chemotaxis in
the BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization
Dose (ND50) is typically 0.8-4 μg/mL in the
presence of 50 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.
Reviewed Applications
Read 1 review rated 4 using AF880 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL19/MIP-3 beta
CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.
References
- Kim, C.H. et al. (1998) J. Immunol. 160:2418.
- Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
- Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
- Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Alternate Names
ELC, MIP-3 beta, MIP3 beta
Gene Symbol
CCL19
UniProt
Additional CCL19/MIP-3 beta Products
Product Documents for Mouse CCL19/MIP-3 beta Antibody
Product Specific Notices for Mouse CCL19/MIP-3 beta Antibody
For research use only
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