CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Chemotaxis Induced by CCL19/MIP-3 beta and Neutralization by Mouse CCR7 Antibody.

Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND₅₀ is typically 2-10 µg/mL.

Detection of CCR7 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR7 Antibody

Application

Recommended Usage

CyTOF-reported

Spitzer, M. et al. (2015) Science 349: 1259425. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse CD3⁺ splenocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed non-stimulated mouse splenocytes

Neutralization

Measured by its ability to neutralize CCL19/MIP-3 beta-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR7. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 25 ng/mL Recombinant Mouse CCL19/MIP-3 beta.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.

Alternate Names

BLR2, CC-CKR-7, CCR7, CD197, CDw197, CMKBR7, EBI1

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Gene Symbol

CCR7

UniProt

NP_031745

Additional CCR7 Products

Product Documents for Mouse CCR7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCR7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CCR7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3477

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Mouse CCR7 Antibody

CCR7 in Mouse Splenocytes.