Mouse CD8 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB116

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Cell depletion, CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition of T Cell Function

Cited:

Cell Selection, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 53-6.7

View all CD8 Antibodies »

Product Specifications

Immunogen

Mouse thymus or spleen

Specificity

Detects both the alpha and alpha' chains of mouse CD8 (1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CD8 alpha Antibody

CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD8 alpha in Mouse Splenocytes.

CD8a was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8a Monoclonal Antibody (Catalog # MAB116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD8a antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD8 alpha in Mouse Splenocytes.

CD8a was detected in mouse splenocytes using Rat Anti-Mouse CD8a Monoclonal Antibody (Catalog # MAB116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CD8 alpha Antibody

Application
Recommended Usage

Cell depletion

Hathcock, K.S. (1991) Current protocols in immunology, pp. 3.4.1. Kruisbeek, A.M. (1991) Current protocols in immunology, pp. 4.1.1.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunoprecipitation

Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.

Inhibition of T Cell Function

This antibody has been used for inhibition of T cell responses to IL-2 (Takahashi, K. et al., 1992, Proc. Natl. Acad. Sci. USA 89:5557 - 5561), MHC class I (Anel, A. et al., 1996, Eur. J. Immunol. 26:2310 - 2319) or antigens (Alexander-Miller, M.A. et al., 1996, J. Exp. Med. 184:485 - 492).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using MAB116 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha/Ly-2 or alpha′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma delta TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References

  1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. Hayakawa, K. et al. (1994) Science 263:1131.
  4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  5. Rocha, B. et al. (1992) Immunol. Today 13:449.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
  8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

Additional CD8 Products

Product Documents for Mouse CD8 alpha Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD8 alpha Antibody

For research use only

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