Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD8a APC-conjugated Monoclonal Antibody (Catalog # FAB116A) or (B) Rat IgG_2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD8 alpha APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha/Ly-2 or alpha′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma delta TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References

Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
Hayakawa, K. et al. (1994) Science 263:1131.
MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
Rocha, B. et al. (1992) Immunol. Today 13:449.
Wang, J. and J.R. Klein (1994) Science 265:1860.
Vermec, D. et al. (1992) J. Exp. Med. 176:47.
Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

Additional CD8 Products

Product Documents for Mouse CD8 alpha APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD8 alpha APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse CD8 alpha APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB116A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD8 alpha APC-conjugated Antibody

Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry.