Mouse CXCR5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6198
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-reported, Flow Cytometry, Immunocytochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 614641
Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCR5
Met1-Pro57
Accession # Q04683
Met1-Pro57
Accession # Q04683
Specificity
Detects recombinant mouse CXCR5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CXCR7 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CXCR5 Antibody
CXCR5 in A20 Mouse Cell Line.
CXCR5 was detected in immersion fixed A20 mouse B cell lymphoma cell line using Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CXCR5 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Detection of CXCR5 in T Follicular Helper Cells by Flow Cytometry.
T follicular helper cells from immunized Balb/c mice were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # MAB6198) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).Applications for Mouse CXCR5 Antibody
Application
Recommended Usage
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes and T follicular helper cells
Sample: Mouse splenocytes and T follicular helper cells
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes and immersion fixed A20 mouse B cell lymphoma cell line
Sample: Immersion fixed mouse splenocytes and immersion fixed A20 mouse B cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR5
Alternate Names
BLR1, CD185, CXCR5
Gene Symbol
CXCR5
UniProt
Additional CXCR5 Products
Product Documents for Mouse CXCR5 Antibody
Product Specific Notices for Mouse CXCR5 Antibody
For research use only
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