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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Cited:

Cell Selection, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 209701

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endoglin
Glu21-Gly581 (predicted)
Accession # NP_031958

Specificity

Detects mouse Endoglin in direct ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant human Endoglin.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Endoglin/CD105 Antibody

Endoglin/CD105 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Endoglin/CD105 in Mouse Embryo.

Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.) using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Image depicts staining at 10X magnification./p>
Endoglin/CD105 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Endoglin/CD105 in Mouse Embryo.

Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.) using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Image depicts staining at 40X magnification./p>
Endoglin/CD105 antibody in Mouse Mesenchymal Stem Cells by Immunocytochemistry (ICC).

Endoglin/CD105 in Mouse Mesenchymal Stem Cells.

Endoglin/CD105 was detected in immersion fixed mouse mesenchymal stem cells using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Endoglin/CD105 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MS-1 mouse pancreatic islet endothelial cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed fixed mouse mesenchymal stem cells, immersion fixed D3 mouse embryonic cell line differentiated to embryoid bodies, and immersion fixed MS-1 mouse pancreatic islet endothelial cell line

Immunohistochemistry

8-25 µg/mL
Sample:

Immersion fixed frozen sections of mouse embryo (13.5 d.p.c.)

 

Western Blot

1 µg/mL
Sample: Recombinant Mouse Endoglin/CD105 Fc Chimera (Catalog # 1320-EN)
under non-reducing conditions only

Reviewed Applications

Read 4 reviews rated 4.5 using MAB1320 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T betaRIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal, and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T betaRII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP-7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T betaRI/ALK5 but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to anti-angiogenic activity (15).

References

  1. Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.

Alternate Names

CD105, ENG

Entrez Gene IDs

2022 (Human); 13805 (Mouse); 497010 (Rat)

Gene Symbol

ENG

UniProt

Additional Endoglin/CD105 Products

Product Documents for Mouse Endoglin/CD105 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Endoglin/CD105 Antibody

For research use only

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