Mouse IFN-alpha / beta R2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1083
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Neutralization, Western Blot
Cited:
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R2
Ser22-Ala239 (Lys160Asn)
Accession # Q9D1R7
Ser22-Ala239 (Lys160Asn)
Accession # Q9D1R7
Specificity
Detects mouse IFN‑ alpha/ beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta, rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IFN-alpha / beta R2 Antibody
Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha/ beta R2 Antibody.
Recombinant Mouse Limitin (1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-a/ beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083). The ND50 is typically 2-10 µg/mL.
Detection of IFN-alpha / beta R2 in A20 cells by Flow Cytometry
A20 cells were stained with Goat Anti-Mouse IFN-alpha / beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse IFN-alpha / beta R2 Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: A20 cells
Sample: A20 cells
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IFN-alpha / beta R2
Sample: Recombinant Mouse IFN-alpha / beta R2
Neutralization
Measured by its ability to neutralize IFN‑ alpha/ beta R2-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha/beta R2
Long Name
Interferon alpha/beta Receptor 2
Alternate Names
IFN-aR2, IFN-bR2, IFNAR2, IFNbR2
Gene Symbol
IFNAR2
UniProt
Additional IFN-alpha/beta R2 Products
Product Documents for Mouse IFN-alpha / beta R2 Antibody
Product Specific Notices for Mouse IFN-alpha / beta R2 Antibody
For research use only
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