Mouse IL-18 R alpha/IL-1 R5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF856
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Neutralization, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, In vivo assay, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha/IL-1 R5
Ser19-Gly326
Accession # Q61098
Ser19-Gly326
Accession # Q61098
Specificity
Detects mouse IL‑18 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL‑18 R is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IL-18 R alpha/IL-1 R5 Antibody
IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Mouse IL‑18 R alpha/IL‑1 R5 Antibody.
In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND50 is typically 0.2-0.8 µg/mL.
Detection of IL-18 R alpha/IL-1 R5 in B6 splenocytes by Flow Cytometry.
B6 splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Goat Anti-Mouse IL-18 R alpha/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse IL-18 R alpha/IL-1 R5 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: B6 splenocytes
Sample: B6 splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL-18 R alpha/IL-1 R5
Sample: Recombinant Mouse IL-18 R alpha/IL-1 R5
Neutralization
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. Ahn, H.J. et al. (1997) J. Immunol. 159:2125. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5
Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL18 in vitro, coexpression of IL-18 R alpha and IL18 R beta is required for high affinity binding and IL-18 responsiveness.
Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
Long Name
Interleukin 18 Receptor alpha
Alternate Names
CD218a, IL-1 R5, IL-1 Rrp, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1
Gene Symbol
IL18R1
UniProt
Additional IL-18 R alpha/IL-1 R5 Products
Product Documents for Mouse IL-18 R alpha/IL-1 R5 Antibody
Product Specific Notices for Mouse IL-18 R alpha/IL-1 R5 Antibody
For research use only
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