Mouse Integrin beta4/CD104 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4054
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 308601
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta4/CD104
Asn29-Ser711
Accession # NP_001005608
Asn29-Ser711
Accession # NP_001005608
Specificity
Detects mouse Integrin beta4/CD104 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Integrin beta1, beta2, beta6, beta7, or recombinant human Integrin beta3.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Integrin beta4/CD104 Antibody
Detection of Mouse Integrin beta4/CD104 by Western Blot.
Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Integrin beta4/CD104 Monoclonal Antibody (Catalog # MAB4054) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Integrin beta4/CD104 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Integrin beta4/CD104 in CMT-93 Mouse Cell Line by Flow Cytometry.
CMT-93 mouse rectal carcinoma cell line was stained with Mouse Integrin beta4/CD104 Monoclonal Antibody (Catalog # MAB4054, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).Applications for Mouse Integrin beta4/CD104 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: CMT‑93 mouse rectal carcinoma cell line
Sample: CMT‑93 mouse rectal carcinoma cell line
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestinal villi
Sample: Perfusion fixed frozen sections of mouse intestinal villi
Western Blot
2 µg/mL
Sample: Mouse small intestine tissue
Sample: Mouse small intestine tissue
Reviewed Applications
Read 4 reviews rated 4.5 using MAB4054 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 4/CD104
Alternate Names
CD104, ITGB4
Gene Symbol
ITGB4
UniProt
Additional Integrin beta 4/CD104 Products
Product Documents for Mouse Integrin beta4/CD104 Antibody
Product Specific Notices for Mouse Integrin beta4/CD104 Antibody
For research use only
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