Mouse Kynureninase Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7389
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 771312
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Kynureninase
Met1-Ser464
Accession # Q9CXF0
Met1-Ser464
Accession # Q9CXF0
Specificity
Detects mouse Kynureninase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human Kynureninase is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Kynureninase Antibody
Detection of Mouse Kynureninase by Western Blot.
Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Kynureninase Monoclonal Antibody (Catalog # MAB7389) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kynureninase in Mouse Liver.
Kynureninase was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse Kynureninase Monoclonal Antibody (Catalog # MAB7389) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Kynureninase Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver
Sample: Perfusion fixed frozen sections of mouse liver
Western Blot
0.5 µg/mL
Sample: Mouse liver tissue
Sample: Mouse liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kynureninase
Alternate Names
KYNU, L-kynurenine hydrolase
Gene Symbol
KYNU
UniProt
Additional Kynureninase Products
Product Documents for Mouse Kynureninase Antibody
Product Specific Notices for Mouse Kynureninase Antibody
For research use only
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