Detects mouse Lgr5/GPR49 in ELISA. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse Lgr5/GPR49 Alexa Fluor® 488-conjugated Antibody

Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either mouse Lgr5/GPR49 (filled histogram) or irrelevant transfectants (open histogram) was stained with Rat Anti-Mouse Lgr5/GPR49 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB8240G). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Lgr5/GPR49 Alexa Fluor® 488-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse Lgr5/GPR49

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Lgr5/GPR49

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5⁺ stem cells can produce all epithelial cell types of the intestinal crypts (4). It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD20" by Peng et al.(1).

References

Peng et al. (2013) Cell Rep. 3(6):1885.
de Lau et al. (2014) Genes Dev. 28(4):305.
Barker et al. (2013) Development 140(12):2484.
Clevers (2013) Nature 495(7439):53.
Wu et al. (2014) Nat Commun 5:3149.
Jang et al. (2013) PLoS One 8(12):e82390495.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Gene Symbol

LGR5

UniProt

Q9Z1P4

Additional Lgr5/GPR49 Products

Product Documents for Mouse Lgr5/GPR49 Alexa Fluor® 488-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Lgr5/GPR49 Alexa Fluor® 488-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Mouse Lgr5/GPR49 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8240G

Key Product Details

Species Reactivity

Validated:

Cited:

Applications