RAW 264.7 mouse monocyte/macrophage cell line was stained with Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

TLR4 in RAW 264.7 Mouse Cell Line.

TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse TLR4 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name

Toll-like Receptor 4

Alternate Names

CD284

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Gene Symbol

TLR4

UniProt

Q9QUK6

Additional TLR4 Products

Product Documents for Mouse TLR4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse TLR4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse TLR4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB27591

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all TLR4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse TLR4 Antibody

Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry.