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Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1037V

R&D Systems, part of Bio-Techne
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FAB1037V-025

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # RB6-8C5

Product Specifications

Immunogen

The immunogen for this antibody is Ly-6G (Gr-1, Gr1).

Specificity

Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody

Detection of Gr-1/Ly-6G antibody in Mouse Bone Marrow Cells antibody by Flow Cytometry.

Detection of Gr‑1/Ly‑6G in Mouse Bone Marrow Cells by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse Integrin aM/CD11b APC-conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Rat Anti-Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1037V) or (B) Rat IgG2BAlexa Fluor 405 Isotype Control (Catalog # IC013V). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Mouse bone marrow cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References

  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Ly-6C, Ly-6G, Ly6G

Entrez Gene IDs

546644 (Mouse)

Gene Symbol

LY6G

Additional Ly-6G/Ly-6C (Gr-1) Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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