Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
Resting skin vessels contain high density HS moieties preferentially expressed at their basolateral compartments.(A, C, E, F, G) Axial and longitudinal sections of vessels of (A, B) naïve frozen, or (C, D, E, F, G) paraffin embedded murine flank skin tissues. Tissues were stained for HS with monoclonal mouse IgM (10E4 epitope; red), goat anti mouse VE-cadherin (cyan), FITC-mouse monoclonal to alpha-SMA (green), and nuclei (blue). (H) HS and alpha-SMA profile of the fluorescence intensity along the purple arrow in the merged image. Double-headed black arrow denotes the vessel lumen as indicated by VE-cadherin. In C, D and G, small-headed arrows denote the endothelial apical side, large-headed arrows denote the endothelial basolateral side, and broken-line arrows denote the pericyte basolateral side. Images were taken at 100X magnification. Scale bar represents 5 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085699), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat VE-Cadherin by Immunohistochemistry
Chronic intermittent hypoxia modifies the BM vascular structure. a’–e’ Representative images of femur bone marrow stained with vWF, CD105, VE-cadherin, SMA, and CD11b counterstained with hematoxylin. a”, c”, d” BM from CIH exposed rats (n = 6) has more VE-cadherin+ vessels and SMA coverage but less vWF+ sinusoids (400×, Leica DM2500). e’, e” Representative images of CD11b immunohistochemistry in femur BM show an increase in BM monocyte count in CIH exposed animals. (400×, Leica DM2500) a’, a”’, b’, b” No changes in the total number of vessels or in megakaryocyte count were observed, as accounted by CD105 and vWF staining, respectively. Results are represented as the mean ± SD of bone marrow sections from six male Wistar rats (*p < 0.05; **p < 0.01). f Representative images of femur bone marrow fluorescently immunostained for VE-cadherin show an increase in total VE-cadherin vessels and in VE-cadherin vessel coverage. Scale bar, 50 μm (insets magnified 2.5×). Images were acquired with a Zeiss LSM 510 META microscope Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26856724), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
Resting skin vessels contain high density HS moieties preferentially expressed at their basolateral compartments.(A, C, E, F, G) Axial and longitudinal sections of vessels of (A, B) naïve frozen, or (C, D, E, F, G) paraffin embedded murine flank skin tissues. Tissues were stained for HS with monoclonal mouse IgM (10E4 epitope; red), goat anti mouse VE-cadherin (cyan), FITC-mouse monoclonal to alpha-SMA (green), and nuclei (blue). (H) HS and alpha-SMA profile of the fluorescence intensity along the purple arrow in the merged image. Double-headed black arrow denotes the vessel lumen as indicated by VE-cadherin. In C, D and G, small-headed arrows denote the endothelial apical side, large-headed arrows denote the endothelial basolateral side, and broken-line arrows denote the pericyte basolateral side. Images were taken at 100X magnification. Scale bar represents 5 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085699), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
Resting skin vessels contain high density HS moieties preferentially expressed at their basolateral compartments.(A, C, E, F, G) Axial and longitudinal sections of vessels of (A, B) naïve frozen, or (C, D, E, F, G) paraffin embedded murine flank skin tissues. Tissues were stained for HS with monoclonal mouse IgM (10E4 epitope; red), goat anti mouse VE-cadherin (cyan), FITC-mouse monoclonal to alpha-SMA (green), and nuclei (blue). (H) HS and alpha-SMA profile of the fluorescence intensity along the purple arrow in the merged image. Double-headed black arrow denotes the vessel lumen as indicated by VE-cadherin. In C, D and G, small-headed arrows denote the endothelial apical side, large-headed arrows denote the endothelial basolateral side, and broken-line arrows denote the pericyte basolateral side. Images were taken at 100X magnification. Scale bar represents 5 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085699), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Flow Cytometry
FACS analysis of emerging vascular EC progenies from mES and iPS cells.Adherent cells (2x105) were detached and subjected to two-step FACS-aided purification. Control FACS profile on day 5 of cells derived from mES (J1) cells (A). Representative FACS profiles of day 5, with vascular progenies assessed using anti-Flk1 and anti-VE-cadherin antibodies obtained from mES (J1) cells (B) and derived from miPS (iMZ-21) cells (C); Control FACS profile on day 5 of cells derived from miPS (iMZ-21) cells (D). Representative FACS after the second step of purification derived from mES (E) and iPS cells (F). The yield of Flk1+VE-cadherin+ after the second round of FACS was 100% for both mES and miPS-derived vascular progenies. Morphology of mES- and miPS-derived vascular ECs (G-J). Flk1+VE-cadherin+ vascular progenies derived from mES and miPS cells were cultured overnight in IV Col-coated dishes, immunostained with anti-VE-cadherin (green) and anti-CD31 (red) of cells derived from mES cells (G&H) and miPS cells (I&J). DAPI, nucleus (blue). Magnifications are as indicated; the scale bar is 200 µm. Experiments were repeated 3 times. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085549), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
Resting skin vessels contain high density HS moieties preferentially expressed at their basolateral compartments.(A, C, E, F, G) Axial and longitudinal sections of vessels of (A, B) naïve frozen, or (C, D, E, F, G) paraffin embedded murine flank skin tissues. Tissues were stained for HS with monoclonal mouse IgM (10E4 epitope; red), goat anti mouse VE-cadherin (cyan), FITC-mouse monoclonal to alpha-SMA (green), and nuclei (blue). (H) HS and alpha-SMA profile of the fluorescence intensity along the purple arrow in the merged image. Double-headed black arrow denotes the vessel lumen as indicated by VE-cadherin. In C, D and G, small-headed arrows denote the endothelial apical side, large-headed arrows denote the endothelial basolateral side, and broken-line arrows denote the pericyte basolateral side. Images were taken at 100X magnification. Scale bar represents 5 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085699), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
Resting skin vessels contain high density HS moieties preferentially expressed at their basolateral compartments.(A, C, E, F, G) Axial and longitudinal sections of vessels of (A, B) naïve frozen, or (C, D, E, F, G) paraffin embedded murine flank skin tissues. Tissues were stained for HS with monoclonal mouse IgM (10E4 epitope; red), goat anti mouse VE-cadherin (cyan), FITC-mouse monoclonal to alpha-SMA (green), and nuclei (blue). (H) HS and alpha-SMA profile of the fluorescence intensity along the purple arrow in the merged image. Double-headed black arrow denotes the vessel lumen as indicated by VE-cadherin. In C, D and G, small-headed arrows denote the endothelial apical side, large-headed arrows denote the endothelial basolateral side, and broken-line arrows denote the pericyte basolateral side. Images were taken at 100X magnification. Scale bar represents 5 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085699), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Flow Cytometry
FACS analysis of emerging vascular EC progenies from mES and iPS cells.Adherent cells (2x105) were detached and subjected to two-step FACS-aided purification. Control FACS profile on day 5 of cells derived from mES (J1) cells (A). Representative FACS profiles of day 5, with vascular progenies assessed using anti-Flk1 and anti-VE-cadherin antibodies obtained from mES (J1) cells (B) and derived from miPS (iMZ-21) cells (C); Control FACS profile on day 5 of cells derived from miPS (iMZ-21) cells (D). Representative FACS after the second step of purification derived from mES (E) and iPS cells (F). The yield of Flk1+VE-cadherin+ after the second round of FACS was 100% for both mES and miPS-derived vascular progenies. Morphology of mES- and miPS-derived vascular ECs (G-J). Flk1+VE-cadherin+ vascular progenies derived from mES and miPS cells were cultured overnight in IV Col-coated dishes, immunostained with anti-VE-cadherin (green) and anti-CD31 (red) of cells derived from mES cells (G&H) and miPS cells (I&J). DAPI, nucleus (blue). Magnifications are as indicated; the scale bar is 200 µm. Experiments were repeated 3 times. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085549), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
FACS analysis of emerging vascular EC progenies from mES and iPS cells.Adherent cells (2x105) were detached and subjected to two-step FACS-aided purification. Control FACS profile on day 5 of cells derived from mES (J1) cells (A). Representative FACS profiles of day 5, with vascular progenies assessed using anti-Flk1 and anti-VE-cadherin antibodies obtained from mES (J1) cells (B) and derived from miPS (iMZ-21) cells (C); Control FACS profile on day 5 of cells derived from miPS (iMZ-21) cells (D). Representative FACS after the second step of purification derived from mES (E) and iPS cells (F). The yield of Flk1+VE-cadherin+ after the second round of FACS was 100% for both mES and miPS-derived vascular progenies. Morphology of mES- and miPS-derived vascular ECs (G-J). Flk1+VE-cadherin+ vascular progenies derived from mES and miPS cells were cultured overnight in IV Col-coated dishes, immunostained with anti-VE-cadherin (green) and anti-CD31 (red) of cells derived from mES cells (G&H) and miPS cells (I&J). DAPI, nucleus (blue). Magnifications are as indicated; the scale bar is 200 µm. Experiments were repeated 3 times. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085549), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Flow Cytometry
FACS analysis of emerging vascular EC progenies from mES and iPS cells.Adherent cells (2x105) were detached and subjected to two-step FACS-aided purification. Control FACS profile on day 5 of cells derived from mES (J1) cells (A). Representative FACS profiles of day 5, with vascular progenies assessed using anti-Flk1 and anti-VE-cadherin antibodies obtained from mES (J1) cells (B) and derived from miPS (iMZ-21) cells (C); Control FACS profile on day 5 of cells derived from miPS (iMZ-21) cells (D). Representative FACS after the second step of purification derived from mES (E) and iPS cells (F). The yield of Flk1+VE-cadherin+ after the second round of FACS was 100% for both mES and miPS-derived vascular progenies. Morphology of mES- and miPS-derived vascular ECs (G-J). Flk1+VE-cadherin+ vascular progenies derived from mES and miPS cells were cultured overnight in IV Col-coated dishes, immunostained with anti-VE-cadherin (green) and anti-CD31 (red) of cells derived from mES cells (G&H) and miPS cells (I&J). DAPI, nucleus (blue). Magnifications are as indicated; the scale bar is 200 µm. Experiments were repeated 3 times. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085549), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat VE-Cadherin by Immunohistochemistry
Chronic intermittent hypoxia modifies the BM vascular structure. a’–e’ Representative images of femur bone marrow stained with vWF, CD105, VE-cadherin, SMA, and CD11b counterstained with hematoxylin. a”, c”, d” BM from CIH exposed rats (n = 6) has more VE-cadherin+ vessels and SMA coverage but less vWF+ sinusoids (400×, Leica DM2500). e’, e” Representative images of CD11b immunohistochemistry in femur BM show an increase in BM monocyte count in CIH exposed animals. (400×, Leica DM2500) a’, a”’, b’, b” No changes in the total number of vessels or in megakaryocyte count were observed, as accounted by CD105 and vWF staining, respectively. Results are represented as the mean ± SD of bone marrow sections from six male Wistar rats (*p < 0.05; **p < 0.01). f Representative images of femur bone marrow fluorescently immunostained for VE-cadherin show an increase in total VE-cadherin vessels and in VE-cadherin vessel coverage. Scale bar, 50 μm (insets magnified 2.5×). Images were acquired with a Zeiss LSM 510 META microscope Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26856724), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
Resting skin vessels contain high density HS moieties preferentially expressed at their basolateral compartments.(A, C, E, F, G) Axial and longitudinal sections of vessels of (A, B) naïve frozen, or (C, D, E, F, G) paraffin embedded murine flank skin tissues. Tissues were stained for HS with monoclonal mouse IgM (10E4 epitope; red), goat anti mouse VE-cadherin (cyan), FITC-mouse monoclonal to alpha-SMA (green), and nuclei (blue). (H) HS and alpha-SMA profile of the fluorescence intensity along the purple arrow in the merged image. Double-headed black arrow denotes the vessel lumen as indicated by VE-cadherin. In C, D and G, small-headed arrows denote the endothelial apical side, large-headed arrows denote the endothelial basolateral side, and broken-line arrows denote the pericyte basolateral side. Images were taken at 100X magnification. Scale bar represents 5 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085699), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Flow Cytometry
FACS analysis of emerging vascular EC progenies from mES and iPS cells.Adherent cells (2x105) were detached and subjected to two-step FACS-aided purification. Control FACS profile on day 5 of cells derived from mES (J1) cells (A). Representative FACS profiles of day 5, with vascular progenies assessed using anti-Flk1 and anti-VE-cadherin antibodies obtained from mES (J1) cells (B) and derived from miPS (iMZ-21) cells (C); Control FACS profile on day 5 of cells derived from miPS (iMZ-21) cells (D). Representative FACS after the second step of purification derived from mES (E) and iPS cells (F). The yield of Flk1+VE-cadherin+ after the second round of FACS was 100% for both mES and miPS-derived vascular progenies. Morphology of mES- and miPS-derived vascular ECs (G-J). Flk1+VE-cadherin+ vascular progenies derived from mES and miPS cells were cultured overnight in IV Col-coated dishes, immunostained with anti-VE-cadherin (green) and anti-CD31 (red) of cells derived from mES cells (G&H) and miPS cells (I&J). DAPI, nucleus (blue). Magnifications are as indicated; the scale bar is 200 µm. Experiments were repeated 3 times. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085549), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
FACS analysis of emerging vascular EC progenies from mES and iPS cells.Adherent cells (2x105) were detached and subjected to two-step FACS-aided purification. Control FACS profile on day 5 of cells derived from mES (J1) cells (A). Representative FACS profiles of day 5, with vascular progenies assessed using anti-Flk1 and anti-VE-cadherin antibodies obtained from mES (J1) cells (B) and derived from miPS (iMZ-21) cells (C); Control FACS profile on day 5 of cells derived from miPS (iMZ-21) cells (D). Representative FACS after the second step of purification derived from mES (E) and iPS cells (F). The yield of Flk1+VE-cadherin+ after the second round of FACS was 100% for both mES and miPS-derived vascular progenies. Morphology of mES- and miPS-derived vascular ECs (G-J). Flk1+VE-cadherin+ vascular progenies derived from mES and miPS cells were cultured overnight in IV Col-coated dishes, immunostained with anti-VE-cadherin (green) and anti-CD31 (red) of cells derived from mES cells (G&H) and miPS cells (I&J). DAPI, nucleus (blue). Magnifications are as indicated; the scale bar is 200 µm. Experiments were repeated 3 times. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085549), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
Resting skin vessels contain high density HS moieties preferentially expressed at their basolateral compartments.(A, C, E, F, G) Axial and longitudinal sections of vessels of (A, B) naïve frozen, or (C, D, E, F, G) paraffin embedded murine flank skin tissues. Tissues were stained for HS with monoclonal mouse IgM (10E4 epitope; red), goat anti mouse VE-cadherin (cyan), FITC-mouse monoclonal to alpha-SMA (green), and nuclei (blue). (H) HS and alpha-SMA profile of the fluorescence intensity along the purple arrow in the merged image. Double-headed black arrow denotes the vessel lumen as indicated by VE-cadherin. In C, D and G, small-headed arrows denote the endothelial apical side, large-headed arrows denote the endothelial basolateral side, and broken-line arrows denote the pericyte basolateral side. Images were taken at 100X magnification. Scale bar represents 5 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085699), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VE-Cadherin by Immunocytochemistry/Immunofluorescence
FACS analysis of emerging vascular EC progenies from mES and iPS cells.Adherent cells (2x105) were detached and subjected to two-step FACS-aided purification. Control FACS profile on day 5 of cells derived from mES (J1) cells (A). Representative FACS profiles of day 5, with vascular progenies assessed using anti-Flk1 and anti-VE-cadherin antibodies obtained from mES (J1) cells (B) and derived from miPS (iMZ-21) cells (C); Control FACS profile on day 5 of cells derived from miPS (iMZ-21) cells (D). Representative FACS after the second step of purification derived from mES (E) and iPS cells (F). The yield of Flk1+VE-cadherin+ after the second round of FACS was 100% for both mES and miPS-derived vascular progenies. Morphology of mES- and miPS-derived vascular ECs (G-J). Flk1+VE-cadherin+ vascular progenies derived from mES and miPS cells were cultured overnight in IV Col-coated dishes, immunostained with anti-VE-cadherin (green) and anti-CD31 (red) of cells derived from mES cells (G&H) and miPS cells (I&J). DAPI, nucleus (blue). Magnifications are as indicated; the scale bar is 200 µm. Experiments were repeated 3 times. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0085549), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of VE-Cadherin in Mouse Heart.
Formalin-fixed paraffin-embedded tissue sections of mouse heart were probed for VE-Cadherin mRNA (ACD RNAScope Probe, catalog #312538; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse VE-Cadherin polyclonal antibody (R&D Systems catalog #
AF1002) at 3ug/mL with 1 hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog #
VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cardiac myocytes.
Detection of Mouse Mouse VE-Cadherin Antibody by Western Blot
Warfarin pretreatment enhances tumor Ad5ROBO4 vector expression.A. Multiorgan immunoblot of vehicle (left lanes) or warfarin (right lanes) pretreated Rag2−/− mice injected with 1.0×1011 vp of Ad5ROBO4. B. Densitometry of A revealed that vehicle pretreatment was associated with robust liver, detectable splenic, and trace to undetectable expression in all other sampled organs. Warfarin pretreatment produced a 2.5-fold increased splenic and a 3-fold decreased liver expression while all other organs still evidenced trace to undetectable expression. C. Immunoblot and densitometry of liver and tumor EGFP, VE-cadherin, and beta-tubulin expression in vehicle (left lanes) or warfarin (right lanes) from the same pretreated, Ad5ROBO4-injected mice as in A and B. EGFP densitometry normalized to VE-cadherin, revealed a 4.7-fold decrease in liver and 2-fold increase in increase KO and SC tumor expression produced by warfarin pretreatment. A–C: representative immunoblots from n = 2 mice from 2 independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24376772), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VE-Cadherin Antibody by Western Blot
Semiquantitative immunoblotting reveals differential Ad5ROBO4 reporter expression in tumor compared to liver.A. Immunoblot of EGFP, VE-cadherin, and beta-tubulin loading controls in tissue protein extracts from hCAR:Rag2−/− mice injected with either Ad5ROBO4, left three lanes, or Ad5CMV, right three lanes. B. and C. Densitometry analysis of Ad5ROBO4 vector EGFP expression normalized to either VE-cadherin or beta-tubulin. D. Densitometry of AdCMV vector EGFP expression. As AdCMV expression was hepatocyte specific, this blot was only normalized to beta-tubulin. A–D: Representative immunoblots from n = 4 mice injected with either Ad5ROBO 4 or Ad5CMV vectors. Li: liver, KO: kidney orthotopic tumor, SC: subcutaneous tumor. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24376772), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VE-Cadherin Antibody by Western Blot
Endogenous ROBO4 upregulation despite lower vascular density in orthotopic and xenograft tumors.A. Immunofluorescence of the vascular endothelium in liver (upper panel) and 786-O human renal cell carcinoma (RCC) subcutaneous xenograft tumor (lower panel). B. Vascular area analysis of liver (Li), kidney orthotopic (KO) tumors, and subcutaneous (SC) xenograft tumors (n = 6 mice analyzed). C. Immunoblot of endogenous ROBO4 and the endothelial cell specific VE-cadherin from liver, kidney orthotopic and subcutaneous xenograft 786-O RCC tumors, and from the derivative 786-O cells grown in culture. D. Densitometry analysis of VE-cadherin/tubulin ratio from C mirrors the vascular area determination in B. E. Densitometry analysis of endogenous ROBO4 normalized to VE-cadherin expression reveals a 1.4- to 2-fold increase in SC and KO tumors compared to liver. C–E: Immunoblot and densitometry was repeated twice with two independent sets of protein extracts from two different tumor-bearing mice with essentially the same results. A. Magnification: 100X, Red: endomucin/CD31 antibody cocktail, Blue: DAPI. B. *p<0.05, one way ANOVA with Tukey's correction, mean ± SD. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24376772), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VE-Cadherin Antibody by Western Blot
Warfarin pretreatment enhances tumor Ad5ROBO4 vector expression.A. Multiorgan immunoblot of vehicle (left lanes) or warfarin (right lanes) pretreated Rag2−/− mice injected with 1.0×1011 vp of Ad5ROBO4. B. Densitometry of A revealed that vehicle pretreatment was associated with robust liver, detectable splenic, and trace to undetectable expression in all other sampled organs. Warfarin pretreatment produced a 2.5-fold increased splenic and a 3-fold decreased liver expression while all other organs still evidenced trace to undetectable expression. C. Immunoblot and densitometry of liver and tumor EGFP, VE-cadherin, and beta-tubulin expression in vehicle (left lanes) or warfarin (right lanes) from the same pretreated, Ad5ROBO4-injected mice as in A and B. EGFP densitometry normalized to VE-cadherin, revealed a 4.7-fold decrease in liver and 2-fold increase in increase KO and SC tumor expression produced by warfarin pretreatment. A–C: representative immunoblots from n = 2 mice from 2 independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24376772), licensed under a CC-BY license. Not internally tested by R&D Systems.
