NHE3/SLC9A3 [p Ser605] Antibody (10A8) [mFluor Violet 450 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-74678MFV450

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Porcine, Opossum, Primate, Rabbit

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 10A8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NHE3/SLC9A3 Antibodies »

Product Summary for NHE3/SLC9A3 [p Ser605] Antibody (10A8) [mFluor Violet 450 SE]

Immunogen

Synthetic peptide made to a region surrounding the Serine 605 portion of rat NHE3. [UniProt# P26433]

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Modification

p Ser605

Specificity

This antibody detects NHE3 posphorylated at serine 605, and does not cross-react with the non-phosphopeptide.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for NHE3/SLC9A3 [p Ser605] Antibody (10A8) [mFluor Violet 450 SE]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NHE3/SLC9A3

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Long Name

Sodium/Hydrogen Exchanger 3

Alternate Names

SLC9A3

Gene Symbol

SLC9A3

Additional NHE3/SLC9A3 Products

Product Documents for NHE3/SLC9A3 [p Ser605] Antibody (10A8) [mFluor Violet 450 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NHE3/SLC9A3 [p Ser605] Antibody (10A8) [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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