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Rat Growth Hormone Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1566

R&D Systems, part of Bio-Techne
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MAB1566
MAB1566-SP

Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 222540

Product Specifications

Immunogen

E. coli-derived recombinant rat Growth Hormone
Phe27-Phe216
Accession # P01244

Specificity

Detects rat Growth Hormone in direct ELISAs and Western blots. Does not cross‑react with recombinant human Growth Hormone.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Rat Growth Hormone Antibody

Detection of Rat Growth Hormone antibody by Western Blot.

Detection of Rat Growth Hormone by Western Blot.

Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Growth Hormone Monoclonal Antibody (Catalog # MAB1566) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Growth Hormone at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Rat Growth Hormone Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone

Growth Hormone, also known as somatotropin, is synthesized primarily in the anterior pituitary. It is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor which is present in many cell types.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Entrez Gene IDs

2688 (Human)

Gene Symbol

GH1

UniProt

Additional Growth Hormone Products

Product Documents for Rat Growth Hormone Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Growth Hormone Antibody

For research use only

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