Siglec-3/CD33 Antibody (HIM3-4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-508
Clone HIM3-4 was used by HLDA to establish CD designation.
![Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]](https://resources.bio-techne.com/images/products/Siglec-3-CD33-Antibody-HIM3-4-Flow-Cytometry-NB500-508-img0003.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Primate
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # HIM3-4
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
NFMY-9s human cell line
Localization
Cell Membrane
Specificity
The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. HLDA V; WS Code M MA112 HLDA VI; WS Code M MA47
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Siglec-3/CD33 Antibody (HIM3-4) - BSA Free
Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]
Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) [NB500-508] - Using the Biotin direct conjugate Surface staining of human peripheral blood leukocytes with anti-human CD33 (HIM3-4) biotin / streptavidin-APC.![Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]](https://resources.bio-techne.com/images/products/Siglec-3-CD33-Antibody-HIM3-4-Flow-Cytometry-NB500-508-img0002.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]")
Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) - BSA Free [NB500-508]
Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) [NB500-508] - Analysis using the PE/Cy5 conjugate of NB500-508. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 K Isotype Control (open histogram) or Anti-Human CD33 PE-Cy5 (filled histogram). Cells in the monocyte gate were used for analysis.Applications for Siglec-3/CD33 Antibody (HIM3-4) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-5 ug/ml
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Siglec-3/CD33
Long Name
Sialic Acid Binding Ig-like Lectin 3
Alternate Names
CD33, gp67, Siglec3
Entrez Gene IDs
945 (Human)
Gene Symbol
CD33
Additional Siglec-3/CD33 Products
Product Documents for Siglec-3/CD33 Antibody (HIM3-4) - BSA Free
Product Specific Notices for Siglec-3/CD33 Antibody (HIM3-4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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